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  • 王敏,汤鼎,王晖,张文艺.混合菌株诱导碳酸钙沉淀修复Cd污染水[J].环境工程学报,2019,13(7):1541-1549.DOI:10.12030/j.cjee.201809132    [点击复制]
  • WANG Min,TANG Ding,WANG Hui,ZHANG Wenyi.Bioremediation of Cd-contaminated water using calcium carbonate precipitation induced by bacterial mixtures[J].,2019,13(7):1541-1549.DOI:10.12030/j.cjee.201809132   [点击复制]
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混合菌株诱导碳酸钙沉淀修复Cd污染水
王敏,汤鼎,王晖,张文艺
0
(常州大学环境与安全工程学院,常州 213164)
摘要:
利用微生物的酶化作用对水体中重金属镉(Cd)进行矿化固定,以减少交换态重金属在环境中的危害;采用X射线衍射(XRD)、扫描电镜(SEM)、傅里叶变换红外谱(FT-IR)等分析测试手段对2株产脲酶矿化菌株(CZW-1和CZW-3)在单一和混合培养体系下生成的矿化产物进行了表征。结果表明,混合培养能提高细菌脲酶活性、提高细菌对Cd的耐受性及对Cd的去除率。单一培养菌株CZW-1和CZW-3的产脲酶活性分别为17.09 U·mL-1和18.23 U·mL-1,对Cd的耐受性为2 mmol·L-1,对Cd的去除率为78.15%、80.32%;混合培养细菌脲酶活性为20.79 U·mL-1,对Cd耐受性为2.5 mmol·L-1,对Cd的去除率为85.50%。3组矿化体系矿化产物均为晶格掺杂、椭球状的CdCO3和CaCO3,但细菌混合体系矿化产物的粒度更大。混合培养体系由于微生物协同作用对于重金属污染修复具有更好的效果。
关键词:  碳酸盐矿化菌  碳酸镉  矿化  共沉淀
DOI:10.12030/j.cjee.201809132
投稿时间:2018-09-20
基金项目:国家自然科学基金资助项目(41302025,41541016)
Bioremediation of Cd-contaminated water using calcium carbonate precipitation induced by bacterial mixtures
WANG Min,TANG Ding,WANG Hui,ZHANG Wenyi
(School of Environmental and Safety Engineering, Changzhou University, Changzhou 213164, China)
Abstract:
In order to reduce the harm of exchangeable fraction of Cd in the environment, the microbial enzymatic method was used to mineralize and fix heavy metal cadmium (Cd) in water. The mineralized products of two urease-producing strains (CZW-1, CZW-3) in single and co-culture methods were characterized by XRD, SEM, EDS, FTIR and particle size analysis. The results showed that the co-culture stains method can improve the urease activity, the tolerance and the removal rates of Cd. The urease activities of single culture strains CZW-1 and CZW-3 were 17.09 U·mL-1, 18.23 U·mL-1, respectively. The tolerance to Cd was 2 mmol·L-1, and the removal rates of Cd were 78.15%, 80.32%. However, the urease activity of the co-culture strains was 20.79 U·mL-1, the tolerance to Cd was 2.5 mmol·L-1, and the removal rate of Cd was 85.5%. The three groups of mineralized products were CdCO3 and CaCO3 with lattice doping and ellipsoid morphology, but the size of mineralized products in the co-culture system was larger. The co-culture stains had a better effect on the remediation of heavy metal contamination due to the synergetic effect of microorganisms in the co-culture system.
Key words:  carbonate mineralizing bacteria  cadmium carbonate  minerlization  coprecipitation