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  • 周小霞,许子牧,胡淑恒,俞志远,蔡秋晨,程诚,沈洁,兰彦.等离子体活化水灭活金黄色葡萄球菌生物膜[J].环境工程学报,2019,13(7):1766-1772.DOI:10.12030/j.cjee.201901156    [点击复制]
  • ZHOU Xiaoxia,XU Zimu,HU Shuheng,YU Zhiyuan,CAI Qiuchen,CHENG Cheng,SHEN Jie,LAN Yan.Inactivation of Staphylococcus aureus biofilm byplasma-activated water[J].,2019,13(7):1766-1772.DOI:10.12030/j.cjee.201901156   [点击复制]
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等离子体活化水灭活金黄色葡萄球菌生物膜
周小霞1,许子牧1,胡淑恒1,俞志远1,蔡秋晨1,程诚2,沈洁2,兰彦2
0
(1.合肥工业大学资源与环境工程学院,合肥 230009;2.中国科学院等离子体物理研究所,合肥 230031)
摘要:
利用大气压空气等离子体诱导生成的活化水(PAW)对金黄色葡萄球菌生物膜的失活效果和机制开展研究。采用菌落形成单位方法对生物膜失活效果进行了评价;采用刃天青荧光染色方法研究了细胞的新陈代谢能力;采用H2- DCFDA荧光染色法检测了细胞内活性氧的浓度;采用分光光度法检测了活化水中具有代表性长寿命活性组分浓度。结果表明,60 min产生的活化水浸泡30 min后,菌落形成单位从107.4减少到102.6;生物膜中约70%的细菌丧失了新陈代谢能力;活化水处理后,生物膜细菌胞内活性氧(ROS)的浓度有明显升高趋势;随着等离子体处理时间的延长,活性基团H2O2NO2-O3的浓度均有不同幅度地提高。综合以上分析,等离子体活化水具有良好的灭菌效果,而且活化水中积累的大量活性基团与细胞内ROS协同作用可能是生物膜失活的主要原因。
关键词:  等离子体活化水  生物膜失活  金黄色葡萄球菌生物膜
DOI:10.12030/j.cjee.201901156
投稿时间:2019-01-24
基金项目:国家自然科学基金资助项目51777206, 51541807, 41372246;安徽省自然科学基金资助项目1708085MB47, 1708085MA13国家自然科学基金资助项目(51777206, 51541807, 41372246);安徽省自然科学基金资助项目(1708085MB47, 1708085MA13);合肥工业大学博士专业研究基金资助项目(JZ2016HGBZ0768, JZ2017HGBZ0944)
Inactivation of Staphylococcus aureus biofilm byplasma-activated water
ZHOU Xiaoxia1,XU Zimu1,HU Shuheng1,YU Zhiyuan1,CAI Qiuchen1,CHENG Cheng2,SHEN Jie2,LAN Yan2
(1.School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China;2.Institute of Plasma Physics, Chinese Academy of Sciences, Hefei 230031, China)
Abstract:
The deactivation effect and antimicrobial mechanism of Staphylococcus aureus biofilm by the plasma activated water (PAW) induced by atmospheric pressure air plasma were studied. The colony forming units (CFU) method was used to estimate the deactivation effect of biofilm, and the resazurin fluorescence staining method was used to study the metabolic rate of cells. The intracellular reactive oxygen and concentration of representative long-life active components in plasma activated water were detected by H2-DCFDA fluorescence staining and spectrophotometry, respectively. The results showed that after 30 minutes immersion in activated water generated in 60 minutes, the number of CFU decreased from 107.4 to 102.6, about 70% bacteria in the biofilm lose their metabolism. The concentration of intracellular reactive oxygen species (ROS) in biofilm significantly increased after plasma activated water treatment. With the extension of plasma treating time, the concentrations of H2O2, NO2- and O3 in plasma activated water increased in varying degrees. Based on the above analysis, the plasma activated water has a good sterilization performance, and the synergistic effect of a large number of active groups accumulated in activated water and intracellular ROS may be the main reason for biofilm inactivation.
Key words:  plasma activated water (PAW)  biofilm inactivation  Staphylococcus aureus biofilm