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Anammox工艺快速启动策略及其微生物特性
杨瑞丽1, 王晓君2,2,2, 吴俊斌1, 郭焱1, 张召基1, 陈少华1
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(1.中国科学院城市环境研究所;2.中国科学院城市环境研究所 城市污染物转化重点实验室)
摘要:
厌氧氨氧化(Anammox)是一种节能、高效、可持续的生物脱氮工艺,其应用受制于Anammox菌缓慢的生长速率及较长的启动周期。本研究探讨种泥投加方式及进水氮负荷提升方式对Anammox反应器快速启动的影响,研究启动过程反应器中微生物丰度及群落结构变化。试验采用先普通活性污泥驯化后接种Anammox种泥的方式启动Anammox工艺,发现在活性迟滞阶段投加Anammox菌种可以加速Anammox工艺的启动。通过缩短水力停留时间增加氮负荷可以避免直接提高进水氮浓度导致的基质毒性抑制,有利于达到更高的总氮去除负荷。稳定运行时反应器的总氮去除负荷达到0.51 kg.m<sub>-3</sub>.d<sub>-1</sub>,Anammox菌丰度为4.92×10<sub>9</sub> copies.(g VSS)<sub>-1</sub>,占总细菌数的2.70%。启动阶段反应器内微生物多样性逐渐下降,检测到浮霉菌门中四个Anammox菌属,以Candidatus Jettenia和Candidatus Kuenenia为主要Anammox菌属。因此,在接种污泥处于Anammox活性迟滞阶段时,结合提升进水氮浓度、缩短水力停留时间和投加富含Anammox菌种泥的方式可以快速启动Anammox反应器。本研究结果可为Anammox实际工程启动提供可借鉴的操作策略。
关键词:  厌氧氨氧化  接种方式  微生物特性  快速启动
DOI:
投稿时间:2018-04-13修订日期:2018-11-13
基金项目:福建省自然科学(2015J05115);中国科学院城市环境研究所青年前沿项目(IUEMS201404)收稿日期录用日期第一
Rapid start-up strategy and microbial characteristics of anammox process
Abstract:
The application of anaerobic ammonium oxidation (Anammox), a cost-effective, efficient and sustainable biological nitrogen removal process, was restricted by slow growth rate of anammox bacteria and longer start-up period. For this study, the effect of inoculation modes and influent nitrogen load on the rapid start-up and microbial community of anammox reactor were investigated during start-up. The anammox process was initiated by inoculating anammox seed sludge after acclimation of activated sludge of wastewater treatment plant. Results showed that adding anammox strain in lag stage was beneficial to the rapid start-up of pilot-scale reactor. A higher nitrogen removal rate (NRR) was achieved via shortening hydraulic retention time an effective mean for avoiding the inhibition of matrix toxicity caused by directly increasing the influent TN concentration. The NRR of 0.51 kg.m<sub>-3</sub>.d<sub>-1</sub>was realized and abundance of anammox bacteria were reached 4.92×10<sub>9</sub> copies.(g VSS)<sub>-1</sub>, accounting for 2.70% of the total bacteria, in the steady running phase. Four Anammox bacteria genus belonging to Planctomycetes were identified, and the dominant genus of functioanl bacteria were Candidatus Jettenia and Candidatus Kuenenia. The anammox process was successfully start-up with short term by inoculating anammox seed sludge, raising influent nitrogen and shorting hydraulic retention time in the lag phase of inoculation sludge. That can be considered as a reference strategy for the practical application of anammox process.
Key words:  Anammox  Inoculation modes  Microorganism characteristics  Rapid start-up